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sos primary antibodies  (Proteintech)


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    Proteintech sos primary antibodies
    Sos Primary Antibodies, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sos primary antibodies/product/Proteintech
    Average 93 stars, based on 13 article reviews
    sos primary antibodies - by Bioz Stars, 2026-03
    93/100 stars

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    Image Search Results


    Inactivation of SERCA2 C674 increases BP and decreases urine output and sodium excretion. (a) BP. * P < .05, significantly different as indicated, n = 10. (b) 24‐hr urine volume and urine sodium excretion, * P < .05, significantly different as indicated, n = 8. (c) Na+/K+‐ATPase activity in primary RPT cells. * P < .05, significantly different as indicated, n = 7. All data are presented as means ± SEM, unpaired Student's t test

    Journal: British Journal of Pharmacology

    Article Title: Inactivation of Cys 674 in SERCA2 increases BP by inducing endoplasmic reticulum stress and soluble epoxide hydrolase

    doi: 10.1111/bph.14937

    Figure Lengend Snippet: Inactivation of SERCA2 C674 increases BP and decreases urine output and sodium excretion. (a) BP. * P < .05, significantly different as indicated, n = 10. (b) 24‐hr urine volume and urine sodium excretion, * P < .05, significantly different as indicated, n = 8. (c) Na+/K+‐ATPase activity in primary RPT cells. * P < .05, significantly different as indicated, n = 7. All data are presented as means ± SEM, unpaired Student's t test

    Article Snippet: Primary antibodies against SERCA2 C674‐SO 3 H (Ying et al., 2008 ; C674‐SO 3 H antibody was a custom polyclonal antibody from Bethyl Laboratories, Inc.), https://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=2970 ( https://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=2970 ; Santa Cruz, Cat# sc‐87099, RRID:AB_2293440), or https://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=214 (Abcam, Cat# ab81296, RRID:AB_2814742) were incubated overnight at 4°C according to the staining procedure of HRP‐DAB kit (CWBIO, Cat# CW0125M).

    Techniques: Activity Assay

    Inactivation of SERCA2 C674 increases intracellular Ca2+ levels in RPT cells. (a) The mRNA levels of both SERCA2a and SERCA2b in the renal cortex. n = 5. (b) SERCA2 protein levels in the renal cortex and primary RPT cells. n = 8. (c) Intracellular Ca2+ detected by Fluo‐4 in primary RPT cells. * P < .05, significantly different as indicated, n = 6. All data are presented as mean ± SEM, unpaired Student's t test

    Journal: British Journal of Pharmacology

    Article Title: Inactivation of Cys 674 in SERCA2 increases BP by inducing endoplasmic reticulum stress and soluble epoxide hydrolase

    doi: 10.1111/bph.14937

    Figure Lengend Snippet: Inactivation of SERCA2 C674 increases intracellular Ca2+ levels in RPT cells. (a) The mRNA levels of both SERCA2a and SERCA2b in the renal cortex. n = 5. (b) SERCA2 protein levels in the renal cortex and primary RPT cells. n = 8. (c) Intracellular Ca2+ detected by Fluo‐4 in primary RPT cells. * P < .05, significantly different as indicated, n = 6. All data are presented as mean ± SEM, unpaired Student's t test

    Article Snippet: Primary antibodies against SERCA2 C674‐SO 3 H (Ying et al., 2008 ; C674‐SO 3 H antibody was a custom polyclonal antibody from Bethyl Laboratories, Inc.), https://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=2970 ( https://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=2970 ; Santa Cruz, Cat# sc‐87099, RRID:AB_2293440), or https://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=214 (Abcam, Cat# ab81296, RRID:AB_2814742) were incubated overnight at 4°C according to the staining procedure of HRP‐DAB kit (CWBIO, Cat# CW0125M).

    Techniques:

    The sustained induction of ER stress by inactivation of SERCA2 C674 accounts for the elevated BP. (a) Representative Western blots of ER stress markers from the renal cortex and quantification of band intensities in graph. Mean ± SEM, unpaired Student's t test, * P < .05, significantly different as indicated, n = 5. (b) Representative Western blots of ER stress markers from primary RPT cells and quantification of band intensities in graph. Mean ± SEM, unpaired Student's t test, * P < .05, significantly different as indicated, p‐PERK, n = 7; BIP, n = 6; ATF6, n = 5; CHOP, n = 6. (c) Na+/K+‐ATPase activity in primary SKI RPT cells treated with 4‐PBA. Mean ± SEM, unpaired Student's t test, * P < .05, significantly different as indicated, n = 5. (d) The effects of 4‐PBA on BP. Mean ± SEM, two‐way ANOVA, * P < .05, significantly different as indicated,; # P < .05, significantly different as indicated, n = 5. (e) The effects of 4‐PBA on SKI urine volume and urine sodium secretion. Mean ± SEM, unpaired Student's t test, * P < .05, significantly different as indicated, n = 5

    Journal: British Journal of Pharmacology

    Article Title: Inactivation of Cys 674 in SERCA2 increases BP by inducing endoplasmic reticulum stress and soluble epoxide hydrolase

    doi: 10.1111/bph.14937

    Figure Lengend Snippet: The sustained induction of ER stress by inactivation of SERCA2 C674 accounts for the elevated BP. (a) Representative Western blots of ER stress markers from the renal cortex and quantification of band intensities in graph. Mean ± SEM, unpaired Student's t test, * P < .05, significantly different as indicated, n = 5. (b) Representative Western blots of ER stress markers from primary RPT cells and quantification of band intensities in graph. Mean ± SEM, unpaired Student's t test, * P < .05, significantly different as indicated, p‐PERK, n = 7; BIP, n = 6; ATF6, n = 5; CHOP, n = 6. (c) Na+/K+‐ATPase activity in primary SKI RPT cells treated with 4‐PBA. Mean ± SEM, unpaired Student's t test, * P < .05, significantly different as indicated, n = 5. (d) The effects of 4‐PBA on BP. Mean ± SEM, two‐way ANOVA, * P < .05, significantly different as indicated,; # P < .05, significantly different as indicated, n = 5. (e) The effects of 4‐PBA on SKI urine volume and urine sodium secretion. Mean ± SEM, unpaired Student's t test, * P < .05, significantly different as indicated, n = 5

    Article Snippet: Primary antibodies against SERCA2 C674‐SO 3 H (Ying et al., 2008 ; C674‐SO 3 H antibody was a custom polyclonal antibody from Bethyl Laboratories, Inc.), https://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=2970 ( https://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=2970 ; Santa Cruz, Cat# sc‐87099, RRID:AB_2293440), or https://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=214 (Abcam, Cat# ab81296, RRID:AB_2814742) were incubated overnight at 4°C according to the staining procedure of HRP‐DAB kit (CWBIO, Cat# CW0125M).

    Techniques: Western Blot, Activity Assay

    Inactivation of SERCA2 C674 up‐regulates sEH expression and down‐regulates expression of D1 receptors. (a) Representative Western blots of sEH and D1 receptors from the renal cortex and quantification of band intensities in graph. * P < .05, significantly different as indicated, sEH, n = 7; D1 receptors, n = 5. (b) Representative Western blots of sEH and D1 receptors from primary RPT cells and quantification of band intensities in graph. * P < .05, significantly different as indicated, sEH, n = 7; D1 receptors, n = 8. (c) Representative picture of immunofluorescence of sEH and D1 receptors in primary RPT cells. Quantification in graph, * P < .05, significantly different as indicated, n = 5. Scale bars = 75 μm. (d) Representative picture of immunohistochemical staining of sEH and D1 receptors in frozen sections of kidney. Quantification in graph, * P < .05, significantly different as indicated, n = 7. Scale bars = 200 μm. All data are presented as mean ± SEM, unpaired Student's t test

    Journal: British Journal of Pharmacology

    Article Title: Inactivation of Cys 674 in SERCA2 increases BP by inducing endoplasmic reticulum stress and soluble epoxide hydrolase

    doi: 10.1111/bph.14937

    Figure Lengend Snippet: Inactivation of SERCA2 C674 up‐regulates sEH expression and down‐regulates expression of D1 receptors. (a) Representative Western blots of sEH and D1 receptors from the renal cortex and quantification of band intensities in graph. * P < .05, significantly different as indicated, sEH, n = 7; D1 receptors, n = 5. (b) Representative Western blots of sEH and D1 receptors from primary RPT cells and quantification of band intensities in graph. * P < .05, significantly different as indicated, sEH, n = 7; D1 receptors, n = 8. (c) Representative picture of immunofluorescence of sEH and D1 receptors in primary RPT cells. Quantification in graph, * P < .05, significantly different as indicated, n = 5. Scale bars = 75 μm. (d) Representative picture of immunohistochemical staining of sEH and D1 receptors in frozen sections of kidney. Quantification in graph, * P < .05, significantly different as indicated, n = 7. Scale bars = 200 μm. All data are presented as mean ± SEM, unpaired Student's t test

    Article Snippet: Primary antibodies against SERCA2 C674‐SO 3 H (Ying et al., 2008 ; C674‐SO 3 H antibody was a custom polyclonal antibody from Bethyl Laboratories, Inc.), https://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=2970 ( https://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=2970 ; Santa Cruz, Cat# sc‐87099, RRID:AB_2293440), or https://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=214 (Abcam, Cat# ab81296, RRID:AB_2814742) were incubated overnight at 4°C according to the staining procedure of HRP‐DAB kit (CWBIO, Cat# CW0125M).

    Techniques: Expressing, Western Blot, Immunofluorescence, Immunohistochemical staining, Staining